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Biomaterials 299 , 122186 (2023)

We found that the ROS detoxification pathway and glutathione metabolism were dysregulated in the ASD brain, pointing to oxidative stress associated with ASD

While GGT5 and GGT1 share 40% of the amino acid sequence (22), GGT5 is not as active in hydrolyzing GSH, GSSG and leukotriene C4 as GGT1 is (29)

Individual fish were divided using a razor blade into 0.5 cm sections and added to a 40 mL glass tube along with 0.1 mL of DI water (18 M-cm Milli-Q, EMD Millipore, Burlington, MA) whereupon tissues were homogenized into a paste using an Omni TH (Omni Int., Kennesaw, GA) tissue homogenizer

Cysteine starvation or xCT blockade confers the opposite result and increases cellular NADPH pools